Thursday, November 24, 2005

NFP--DNA patterning

Preparation of a DNA solution
  • 50 µl of PBS added to DNA powder (thiol-modified DNA with the disulfide bonding cleaved) := solution A
  • 2 µl out of the solution A := solution B
  • Solution B + 18 µl of PBS := solution C
  • Solution C + 10 µl of the cocktail.

Tip #8 of the chip 1 prepared on 112205 was used for the patterning.

3x3 [1,0.5,0.25s] => 6x6 @ (-40,40)

Arrays of dots in a good shape were obtained.