First trial (s/n f112505a):
- 2 µl of the DNA + 10 µl of the cocktail
- 3x3 [2, 1, 0.5s] => 6x6 @ (-40,40)
- Tip #7 or #8 of chip 2 out of the 11-25-05 set was used.
- 79 °F, 83%
- Dot array was obtained. But the diameter, being ~500 nm, seemed to be smaller than that of yesterday's experiments.
Second trial (f112505b):
- Additional 10 µl of the cocktail into the soluion of the first trial. It was attempted to get higher diffusion rate by diluting the solution
- The same gold substrate was used, but with writing in the upper area.
- 3x3 [4, 2, 1s] => 6x6 @ (-40,40)
- The same tip, tip #7 of chip 2 from the 11-25-05 set, was used. The chip is kept in the box F after writing.
- This substrate was subsequently processed for hybridization with target and probe DNAs.
- Target DNA was diluted with PBS and incubated for ~40 min at 37 °C.
- Probe DNA was diluted with PBS and incubated for ~40 min at 37 °C.
Third trial (f112505c):
- The chip used yesterday was hired again. Tip 8 wrote patterns.
- Patterning on a new gold substrate
- 3x3 [6, 4, 2s] => 6x6 @ (-40,40)
- The dot diameters are not as big as the ones obtained yesterday. The yesterday's solutions seems to provide a good condition for larger feature size.
