Glycerol

• Mix with H₂O.
• For protein storage, we use 20% ~ 50%. 20% resists freezing at -20 °C, 50% at -70 °C.
• For cell membrane disruption and simultaneous preservation and most cellular components (protein, DNA). We incubate in 20% at -20 °C for 2 hours ~ overnight.

TX-100

• Suspending MWCNTs. 0.5% TX-100/H2O or PBS. Worked very well. 1% is also fine.
• May sonicate.
• Stock x20 "viscous". 10% TX-100 (4 °C)

Antibody

• Primary Ab: Goat anti-Human VIM IgG.
• Secondary Ab: Gold (10 nm)-Rabbit anti-Goat IgG.
• 1:50 in PBS.
• If necessary, can mix glycerol (~5%).
• Gentle pipetting to avoid forming bubbles.